THE 2-FDCK HCL DIARIES

The lab states fixation will cause the dyes to leak through the cells. I do think the belief is that fixation may possibly injury cell membranes. I did find a paper which describes fixation using methanol or acetic acid, just after staining having a DCFDA derivative. DCFDA is the reagent that stains ROS inside the kit ab113851, and I suspect that i